The term aseptic refers to something an item or environment that’s free of disease or disease-causing microbes.Īseptic techniques are preventative techniques. When you’re working with things that can’t be completely sterilized, you’ll need to use aseptic technique instead. As soon as an item is exposed to unfiltered air, for example, it’s no longer sterile. True sterile fields are very difficult to maintain outside of an autoclave, laboratory hood, or enclosed package. This lowers the risk of infection after a piercing. Professional piercers also often use autoclaves to sterilize their equipment and jewelry. All three combine in an autoclave, a machine that’s used to kill microbes on medical or scientific instruments. Heat, pressure, and steam are the most common sanitization methods. The most common ways to achieve sterility include: Sterile technique involves the sanitization of surfaces and implements. It also extends to more resistant bacterial spores. This includes the standard bacteria, viruses, fungi, and protists. When something is sterile, it’s completely free of any live microscopic organisms. Keep reading for a summary of what you need to know to avoid biocontamination. In this guide, we’ll go over when to use aseptic techniques, when to use sterile techniques, and the differences between each of them. Both work to prevent the spread of disease, but they do so in different ways and aren’t always useful in the same environments. sterile technique.Ĭontrary to popular belief, they aren’t quite identical terms. One of these common points of confusion is the difference between aseptic technique vs. Perform your experiments as rapidly as possible to minimize contamination.Biological and medical glossaries are full of ambiguous language and similar terminology.Be careful not to talk, sing, or whistle when you are performing sterile procedures.Use only sterile glassware and other equipment.If you remove a cap or cover, and have to put it down on the work surface, place the cap with opening facing down.Return the cover as soon as you are finished. until the instant you are ready to use it and never leave it open to the environment. Never uncover a sterile flask, bottle, petri dish, etc.Always cap the bottles and flasks after use and seal multi-well plates with tape or place them in resealable bags to prevent microorganisms and airborne contaminants from gaining entry.Do not unwrap sterile pipettes until they are to be used. Use sterile glass or disposable plastic pipettes and a pipettor to work with liquids, and use each pipette only once to avoid cross contamination.Avoid pouring media and reagents directly from bottles or flasks.Wipe the outside of the containers, flasks, plates, and dishes with 70% ethanol before placing them in the cell culture hood.Always wipe your hands and your work area with 70% ethanol.Leave the cell culture hood running at all times, turning it off only when they will not be used for extended periods of time. Using a Bunsen burner for flaming is not necessary nor is it recommended in a cell culture hood.You may use ultraviolet light to sterilize the air and exposed work surfaces in the cell culture hood between uses.For routine cleaning, wipe the work surface with 70% ethanol before and during work, especially after any spillage.Before and after use, the work surface should be disinfected thoroughly, and the surrounding areas and equipment should be cleaned routinely.The work surface should be uncluttered and contain only items required for a particular procedure it should not be used as a storage area.The cell culture hood should be properly set up and be located in an area that is restricted to cell culture that is free from drafts from doors, windows, and other equipment, and with no through traffic.The simplest and most economical way to reduce contamination from airborne particles and aerosols (e.g., dust, spores, shed skin, sneezing) is to use a cell culture hood.
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